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subconfluent scc15 cells  (ATCC)


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    ATCC subconfluent scc15 cells
    FIG. 1. Time-dependent degradation of E-cadherin during incubation of oral epithelial cells with C. albicans. <t>SCC15</t> cells were exposed to C. albicans strain SC5314 for 2 h (lane 2), 4 h (lane 4), 6 h (lane 6), 8 h (lane 9), 10 h (lane 11), 12 h (lane 13), and 14 h (lane 15), and E-cadherin expression was analyzed by Western blotting. Membranes were probed with -actin to control for sample loading. Cell lysates of C. albicans alone were analyzed to determine E-cadherin and -actin contents (lane 7). Densitometry values (in optical density [OD] units) are indicated below the bands. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experiments are shown in each panel.
    Subconfluent Scc15 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p"

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p

    Journal: Infection and Immunity

    doi: 10.1128/iai.00054-07

    FIG. 1. Time-dependent degradation of E-cadherin during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for 2 h (lane 2), 4 h (lane 4), 6 h (lane 6), 8 h (lane 9), 10 h (lane 11), 12 h (lane 13), and 14 h (lane 15), and E-cadherin expression was analyzed by Western blotting. Membranes were probed with -actin to control for sample loading. Cell lysates of C. albicans alone were analyzed to determine E-cadherin and -actin contents (lane 7). Densitometry values (in optical density [OD] units) are indicated below the bands. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experiments are shown in each panel.
    Figure Legend Snippet: FIG. 1. Time-dependent degradation of E-cadherin during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for 2 h (lane 2), 4 h (lane 4), 6 h (lane 6), 8 h (lane 9), 10 h (lane 11), 12 h (lane 13), and 14 h (lane 15), and E-cadherin expression was analyzed by Western blotting. Membranes were probed with -actin to control for sample loading. Cell lysates of C. albicans alone were analyzed to determine E-cadherin and -actin contents (lane 7). Densitometry values (in optical density [OD] units) are indicated below the bands. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experiments are shown in each panel.

    Techniques Used: Incubation, Expressing, Western Blot, Control

    FIG. 3. Analysis of E-cadherin gene expression during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for up to 14 h, and E-cadherin gene expression was analyzed by quantitative RT-PCR. Data were expressed as fold induction values obtained by dividing the relative expression value for E-cadherin in epithelial cells cocultured with C. albicans by the relative expression value for E-cadherin in epithelial cells alone. A fold induc- tion value of 1.0 indicates that there was no induction of E-cadherin expression in epithelial cells by C. albicans, while values less than 0.5 and greater that 2.0 indicate that there was decreased and increased E-cadherin expression in epithelial cells cocultured with C. albicans. The error bars indicate one standard deviation of the mean of tripli- cate experiments.
    Figure Legend Snippet: FIG. 3. Analysis of E-cadherin gene expression during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for up to 14 h, and E-cadherin gene expression was analyzed by quantitative RT-PCR. Data were expressed as fold induction values obtained by dividing the relative expression value for E-cadherin in epithelial cells cocultured with C. albicans by the relative expression value for E-cadherin in epithelial cells alone. A fold induc- tion value of 1.0 indicates that there was no induction of E-cadherin expression in epithelial cells by C. albicans, while values less than 0.5 and greater that 2.0 indicate that there was decreased and increased E-cadherin expression in epithelial cells cocultured with C. albicans. The error bars indicate one standard deviation of the mean of tripli- cate experiments.

    Techniques Used: Gene Expression, Incubation, Quantitative RT-PCR, Expressing, Standard Deviation

    FIG. 4. E-cadherin is proteolytically degraded by C. albicans. (A) Effect of inhibitors of proteinases on E-cadherin degradation dur- ing incubation of SCC15 cells with C. albicans strain SC5314. (B) Effect of C. albicans strain SC5314 and C. albicans-conditioned medium on the integrity of E-cadherin expressed on epithelial cell-derived mem- branes. Trypsin was used as a positive control. (C) Effect of C. albicans strain SC5314 on the integrity of immunoprecipitated E-cadherin in the presence or absence of a cocktail of protease inhibitors or pepsta- tin A. The protease inhibitor cocktail contained aprotinin, bestatin, leupeptin, E-64, and pepstatin A. E-cadherin integrity was analyzed by Western blotting. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experi- ments are shown in each panel.
    Figure Legend Snippet: FIG. 4. E-cadherin is proteolytically degraded by C. albicans. (A) Effect of inhibitors of proteinases on E-cadherin degradation dur- ing incubation of SCC15 cells with C. albicans strain SC5314. (B) Effect of C. albicans strain SC5314 and C. albicans-conditioned medium on the integrity of E-cadherin expressed on epithelial cell-derived mem- branes. Trypsin was used as a positive control. (C) Effect of C. albicans strain SC5314 on the integrity of immunoprecipitated E-cadherin in the presence or absence of a cocktail of protease inhibitors or pepsta- tin A. The protease inhibitor cocktail contained aprotinin, bestatin, leupeptin, E-64, and pepstatin A. E-cadherin integrity was analyzed by Western blotting. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experi- ments are shown in each panel.

    Techniques Used: Incubation, Derivative Assay, Positive Control, Immunoprecipitation, Protease Inhibitor, Western Blot

    Related Articles

    Incubation:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
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    Expressing:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
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    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
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    Western Blot:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
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    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
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    Control:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Gene Expression:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Quantitative RT-PCR:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Standard Deviation:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Derivative Assay:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Positive Control:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Immunoprecipitation:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Protease Inhibitor:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Infection:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Mutagenesis:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Comparison:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Activity Assay:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Sandwich ELISA:

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p
    Article Snippet: To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the integrity of the monolayer and to determine its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.MDCK cells are known to form tight junctions, which are relatively impermeable to dextran, under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described ( 3 ).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.

    Article Title: Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
    Article Snippet: To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).To confirm the monolayer integrity and test its permeability to macromolecules in vitro, the dextran permeability assay was used, as previously described (3).. The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.. MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.MDCK cells are known to form tight junctions which are relatively impermeable to dextran under these in vitro conditions.



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    FIG. 1. Time-dependent degradation of E-cadherin during incubation of oral epithelial cells with C. albicans. <t>SCC15</t> cells were exposed to C. albicans strain SC5314 for 2 h (lane 2), 4 h (lane 4), 6 h (lane 6), 8 h (lane 9), 10 h (lane 11), 12 h (lane 13), and 14 h (lane 15), and E-cadherin expression was analyzed by Western blotting. Membranes were probed with -actin to control for sample loading. Cell lysates of C. albicans alone were analyzed to determine E-cadherin and -actin contents (lane 7). Densitometry values (in optical density [OD] units) are indicated below the bands. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experiments are shown in each panel.
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    FIG. 1. Time-dependent degradation of E-cadherin during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for 2 h (lane 2), 4 h (lane 4), 6 h (lane 6), 8 h (lane 9), 10 h (lane 11), 12 h (lane 13), and 14 h (lane 15), and E-cadherin expression was analyzed by Western blotting. Membranes were probed with -actin to control for sample loading. Cell lysates of C. albicans alone were analyzed to determine E-cadherin and -actin contents (lane 7). Densitometry values (in optical density [OD] units) are indicated below the bands. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experiments are shown in each panel.

    Journal: Infection and Immunity

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p

    doi: 10.1128/iai.00054-07

    Figure Lengend Snippet: FIG. 1. Time-dependent degradation of E-cadherin during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for 2 h (lane 2), 4 h (lane 4), 6 h (lane 6), 8 h (lane 9), 10 h (lane 11), 12 h (lane 13), and 14 h (lane 15), and E-cadherin expression was analyzed by Western blotting. Membranes were probed with -actin to control for sample loading. Cell lysates of C. albicans alone were analyzed to determine E-cadherin and -actin contents (lane 7). Densitometry values (in optical density [OD] units) are indicated below the bands. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experiments are shown in each panel.

    Article Snippet: The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.

    Techniques: Incubation, Expressing, Western Blot, Control

    FIG. 3. Analysis of E-cadherin gene expression during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for up to 14 h, and E-cadherin gene expression was analyzed by quantitative RT-PCR. Data were expressed as fold induction values obtained by dividing the relative expression value for E-cadherin in epithelial cells cocultured with C. albicans by the relative expression value for E-cadherin in epithelial cells alone. A fold induc- tion value of 1.0 indicates that there was no induction of E-cadherin expression in epithelial cells by C. albicans, while values less than 0.5 and greater that 2.0 indicate that there was decreased and increased E-cadherin expression in epithelial cells cocultured with C. albicans. The error bars indicate one standard deviation of the mean of tripli- cate experiments.

    Journal: Infection and Immunity

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p

    doi: 10.1128/iai.00054-07

    Figure Lengend Snippet: FIG. 3. Analysis of E-cadherin gene expression during incubation of oral epithelial cells with C. albicans. SCC15 cells were exposed to C. albicans strain SC5314 for up to 14 h, and E-cadherin gene expression was analyzed by quantitative RT-PCR. Data were expressed as fold induction values obtained by dividing the relative expression value for E-cadherin in epithelial cells cocultured with C. albicans by the relative expression value for E-cadherin in epithelial cells alone. A fold induc- tion value of 1.0 indicates that there was no induction of E-cadherin expression in epithelial cells by C. albicans, while values less than 0.5 and greater that 2.0 indicate that there was decreased and increased E-cadherin expression in epithelial cells cocultured with C. albicans. The error bars indicate one standard deviation of the mean of tripli- cate experiments.

    Article Snippet: The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.

    Techniques: Gene Expression, Incubation, Quantitative RT-PCR, Expressing, Standard Deviation

    FIG. 4. E-cadherin is proteolytically degraded by C. albicans. (A) Effect of inhibitors of proteinases on E-cadherin degradation dur- ing incubation of SCC15 cells with C. albicans strain SC5314. (B) Effect of C. albicans strain SC5314 and C. albicans-conditioned medium on the integrity of E-cadherin expressed on epithelial cell-derived mem- branes. Trypsin was used as a positive control. (C) Effect of C. albicans strain SC5314 on the integrity of immunoprecipitated E-cadherin in the presence or absence of a cocktail of protease inhibitors or pepsta- tin A. The protease inhibitor cocktail contained aprotinin, bestatin, leupeptin, E-64, and pepstatin A. E-cadherin integrity was analyzed by Western blotting. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experi- ments are shown in each panel.

    Journal: Infection and Immunity

    Article Title: Mucosal Tissue Invasion by Candida albicans Is Associated with E-Cadherin Degradation, Mediated by Transcription Factor Rim101p and Protease Sap5p

    doi: 10.1128/iai.00054-07

    Figure Lengend Snippet: FIG. 4. E-cadherin is proteolytically degraded by C. albicans. (A) Effect of inhibitors of proteinases on E-cadherin degradation dur- ing incubation of SCC15 cells with C. albicans strain SC5314. (B) Effect of C. albicans strain SC5314 and C. albicans-conditioned medium on the integrity of E-cadherin expressed on epithelial cell-derived mem- branes. Trypsin was used as a positive control. (C) Effect of C. albicans strain SC5314 on the integrity of immunoprecipitated E-cadherin in the presence or absence of a cocktail of protease inhibitors or pepsta- tin A. The protease inhibitor cocktail contained aprotinin, bestatin, leupeptin, E-64, and pepstatin A. E-cadherin integrity was analyzed by Western blotting. The positions of the molecular mass markers are indicated on the left. The results of one of three independent experi- ments are shown in each panel.

    Article Snippet: The negative and positive controls included membranes without cells, membranes with subconfluent SCC15 cells, and membranes with confluent MDCK cell (ATCC CCL-34) monolayers.

    Techniques: Incubation, Derivative Assay, Positive Control, Immunoprecipitation, Protease Inhibitor, Western Blot